Small decreases in Ca2+ influx following exposure to JZL184 in isolated rat islets and INS-1 cells.

(A.) Islets were brought from 3 mM glucose to 15 mM glucose with or without 10 μM JZL184 and cytosolic Ca2+ was documented using the probe fura-2 (n = 4). (B) INS-1 cells were brought from 2 mM glucose plus 0.25 mM DZ to 8 mM glucose with 0.25 mM DZ and 30 mM KCl with or without 10 μM JZL184 (n = 3). Ca2+ levels are expressed as the fura-2 ratio at 340/380 nm or the increment above basal in response to treatment (peak and sustained).