LMAS Test Dataset - BMock12 Community Standards

The twelve bacterial replicons of the BMock12 Community Standards were used as reference. It includes the following strains: Species Sample ID Depth of Coverage (x) Muricauda sp. ES.050 2615840527 618.76 Thioclava sp. ES.032 2615840533 78.32 Cohaesibacter sp. ES.047 2615840601 170.59 Propionibacteriaceae bacterium 2615840646 31.90 Marinobacter sp. LV10R510-8 2615840697 447.83 Marinobacter sp. LV10MA510-1 2616644829 135.05 Psychrobacter sp. LV10R520-6 2617270709 425.47 Micromonospora echinaurantiaca 2623620557 14.91 Micromonospora echinofusca 2623620567 18.19 Micromonospora coxensis 2623620609 0.02 Halomonas sp. HL-4 2623620617 507.08 Halomonas sp. HL-93 2623620618 579.87 The raw sequence data of the mock communities, with an even distribution of species, is available at: SRR8073716 For the sake of a less resource-intense evaluation we downsampled to have 20% of the reads available in the original sample and further processed only these. The main challenges of this data set are possibly to assemble the genomes of the three <em>Micromonospora</em> spp. and the two <em>Halomonas</em> spp. strains, which have an ANIb &gt;0.84 and 0.98, respectively. The two <em>Marinobacter</em> spp. have an ANIb of 0.78.