Gene expression NHX1 and SOS1 in Salicornia under different salinty levels

The expression of the S. europaea genes NHX1 and SOS1 in different saline treatments was analysed using quantitative reverse transcriptase polymerase chain reaction (RT–qPCR). The analysed NHX1 and SOS1 genes encode a tonoplast Na+/H+ antiporter and a plasma membrane Na+ /H+ antiporter, respectively. The data contained in this file correspond to S. europaea population collected in Inowrocław, PolandGENERAL INFORMATIONTitle of Dataset: Salicornia qPCR SOS1 and NHX1Author/Principal Investigator InformationName: Stefany Cardenas PerezORCID:https://orcid.org/0000-0003-3919-3498Institution: Department of Geobotany and Landscape Planning, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in TorunAddress: Lwowska 1, 87-100 Torun, Poland Email: [e-mail]-investigator InformationName: Agnieszka PiernikORCID:https://orcid.org/0000-0002-0800-5266Institution: Department of Geobotany and Landscape Planning, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in TorunAddress: Lwowska 1, 87-100 Torun, Poland Email: [e-mail] of data collection: 02.2023 Geographic location of data collection: PolandInformation about funding sources that supported the collection of the data: National Science Center 2021/43/D/NZ8/01137SHARING/ACCESS INFORMATIONLicenses/restrictions placed on the data: CC0DATA & FILE OVERVIEW1. Salicornia qPCR SOS1 and NHX1METHODOLOGICAL INFORMATIONThe reverse transcription reaction, the RNA was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, US). According to the manufacturer’s protocol, cDNA was synthesized from 1.5 µg of total RNA using a mixture of 0.25 µg oligo(dT)20 primer and 0.2 µg of random hexamers with an NG dART RT Kit (Eurx, Gdansk, ´ Poland). The reaction was performed at 25 ◦C for 10 min, followed by 50 ◦C for 50 min. The cDNA was stored at − 20 ◦C. The PCR reaction mixture includes 4 µl of 1/20 diluted cDNA, 0.5 µM gene-specifc primers (Cardenas-Perez et al. (2022) DATA-SPECIFIC INFORMATION FOR: Salicornia qPCR SOS1 and NHX1Number of variables: 6 (salinity treatments)Number of cases: 4 repetitions for each case