Host Factor Experiment (SCL006-R)
收藏DataCite Commons2020-07-23 更新2024-07-13 收录
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https://www.viprbrc.org/brc/svExperimentDetails.spg?expAccession=IRD_SV_SCL006-R&decorator=corona&context=1593107861727
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资源简介：
Purpose: To obtain samples for transcriptional analysis and proteomics in triplicate using wild typeicSARS urbani and icSARS Bat SRBD mutant in 2B4 cells/sorted Calu3 cells with high ACE2 expression. Overview of Experiment: Time Points = 0, 7, 12, 24, 30, 36, 48, 54, 60 and 72 hrs post infection. (Note: there is no time point at 7 h for icSARS Bat SRBD.) Done in triplicate for both RNA and Protein. Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) was used for the mock infections. Infection was done at an MOI of 1.
提供机构：
Virus Pathogen Resource
创建时间：
2020-06-25
