Genomic and transcriptomic profiles underlying the effect of SETD1A disruption on human neurodevelopmental trajectories

To examine the transcriptomic alteration by SETD1A deficiency, combining CRISPR/CAS9 genome editing and 3-Dimentional Cell Culture technology, we generated organoid models of the developing human cerebral cortex derived from isogenic human induced pluripotent stem cells (hiPSCs) modified to carry a SETD1A LoF schizophrenia risk mutation. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT and mutant cortical neurons at DIV70. Comparative gene expression profiling analysis of RNA-seq data for sorted GFP positive neurons of WT and mutant forebrain organoids at DIV70