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Binding proteins for the GA-modified proteins in mouse serum.

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We determined if the glycolaldehyde (GA)-modified proteins could show an affinity with serum proteins. The mouse serum was incubated with the ligand-coupled beads for 1 h at room temperature. The ligands used were BSA and GA-treated BSA (GA-BSA). The proteins bound to the beads were eluted and separated by SDS-PAGE. Seven unique bands detected in the pull-down with the GA-BSA ligands were processed for tryptic digestion and LC-MS/MS analysis.
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2020-04-08
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