Dysregulation of adipocytokines regulated by hsa-miR-548ay-3p/PPAR? signaling pathway leaded to cardiac fibrosis

Cardiac fibrosis is the key to the onset of atrial fibrillation (AF), but the mechanism is not fully understood. Our previous study found that fibrosis-related adipocytokines were dysregulated in epicardial adipose tissue (EAT) from patients with AF. In this study, we explored the factor that induce adipocytokines dysregulation and the mechanism underlying adipocytokines dysregulation-induced cardiac fibrosis. Overall design: In part 1, cardiac fibroblasts(CFs) were treated with conditioned medium (CM) of adipocyte infected with hsa-miR-548ay overexpression lentivirus or vector lentivirus. In part 2, CFs were treated with TGF-beta1 and CM of adipocyte infected with hsa-miR-548ay inhibition lentivirus or vector lentivirus.