ILT4 regulates cytokines during sepsis

Serum TNF-α showed a one-way trend peaking at 6 h after CLP (53.13 ± 5.49 vs. 24.50 ± 4.57, + 117%, p < 0.05) that reverted to preoperative levels 12 h after CLP in WT mice. ILT4 knockout showed no significant impact of CLP on TNF-α levels (50.88 ± 6.38 vs. 53.13 ± 5.49, − 4%, p > 0.05). Twenty-four hours after CLP, serum IL-1β was significantly elevated in the WT mice (3639.13 ± 627. 20 vs. 581.75 ± 152.89, + 525%, p < 0.05) while serum IL-1β was not affected in ILT4−/− mice (3144.63 ± 549.74 vs. 3639.13 ± 627.20, − 14%, p > 0.05). IL-6 was significantly elevated 24 h after CLP in the WT mice (470.75 ± 88.03 vs. 54.25 ± 20.04, + 772%, p < 0.05). The IL-6 levels were significantly lower in ILT4−/− mice compared with those in WT mice after CLP (241.25 ± 45.10 vs. 470.75 ± 88.03, − 49%, p < 0.05). Serum IL-12 levels were significantly high 12 h after CLP in WT mice compared with controls (3508.99 ± 326.77 vs. 1641.57 ± 314.13, + 114%, p < 0.05) but was not significantly affected by ILT4 knockout (3198.74 ± 221.12 vs. 3508.99 ± 326.77, − 9%, p > 0.05)