POSERS: Steganography-Driven Molecular Tagging Using Randomized DNA Sequences

Counterfeiting poses a significant challenge across multiple industries, leading to financial losses and health risks. While DNA-based molecular tagging has emerged as a promising anti-counterfeiting strategy, existing methods rely on predefined DNA sequences, making them vulnerable to replication as sequencing and synthesis technologies advance. To address these limitations, we introduce POSERS (Position-Oriented Scattering of Elements among a Randomized Sequence), a novel steganographic tagging system embedded within DNA sequences. POSERS ensures copy- and forgery-proof authentication by adding restrictions within randomized DNA libraries, enhancing security against counterfeiting attempts. The POSERS design allows the complexity of the libraries to be adjusted based on the customer's needs while ensuring they withstand the ongoing improvements in DNA synthesis and sequencing technologies. We mathematically validate its security properties and experimentally demonstrate its effectiveness using Next-Generation Sequencing (NGS) and an authentication test, successfully distinguishing genuine POSERS tags from counterfeit ones. Our results highlight the potential of POSERS as a long-term, adaptable solution for secure product authentication.The information about the sequencing sample is provided in the supplementary table 5.