Harr Gupta et al. raw data

Bleomycin was intratracheally delivered to mouse lungs to induce fibrosis. At different time points post-bleomycin, precision-cut lung slices (PCLS) from fibrotic mouse lungs were incubated with radiolabeled peptide ([⁶⁴Cu]Cu-PEG-FUD) and radioactivity was quantified via gamma counting and autoradiography. Alternatively, at different time points post-bleomycin, fluorescently labeled peptide (Cy5-PEG-FUD) was delivered to mice subcutaneously, followed by relevant organ <i>ex vivo</i> IVIS fluorescence imaging and signal quantification. Additionally, lung sections were stained with Mason's trichrome staining and subsequently subjected to Ashcroft scoring to determine the level of fibrotic burden.