Overexpression of<b> </b>the transcriptional activators Mxr1 and Mit1 enhances lactic acid production on methanol in <i>Komagataella phaffii</i>

The presence of lactic acid has a repressive effect on methanol utilizing genes in <i>Komagataella phaffii</i>Overexpression of the transcription factor genes <i>MXR1</i> and <i>MIT1</i> leads to higher expression of <i>AOX1</i> and <i>FDH1</i> and improves lactic acid production on MeOH in bioreactor cultivationsKeeping MeOH concentration at 2 % is beneficial for lactic acid production乳酸（lactic acid）对<i>Komagataella phaffii</i>（毕赤酵母）的甲醇利用基因具有抑制作用。转录因子基因<i>MXR1</i>与<i>MIT1</i>的过表达，可显著上调<i>AOX1</i>（醇氧化酶1）与<i>FDH1</i>（甲酸脱氢酶1）的表达水平，并在生物反应器培养中提升以甲醇（MeOH）为底物的乳酸合成效率。将甲醇（MeOH）浓度维持在2%时，有利于乳酸的合成与生产。