In vitro assay to evaluate APC-mediated maintenance of Foxp3 expression by CD4+CD25+ T cells.
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CD4+CD25+ T cells from 12–15 week-old B6 mice were cultured overnight in multiple wells, either alone or with B6 irradiated T-cell depleted spleen cells (APC) and anti-CD3 antibody. Cells were pooled and labeled with anti-CD4, CD25 and anti-Foxp3 antibodies. CD4+CD25+ T cells and APC were pooled from 3 mice. Cells were analyzed for Foxp3 expression by FACS® after gating on CD4+CD25+ cells. Representative results from one of two experiments are shown.
创建时间：
2015-12-02
