Krüppel-associated boxes are potent transcriptional repression domains.
收藏PubMed Central1994-05-10 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC43815/
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资源简介：
The Krüppel-associated box (KRAB) is a highly conserved, 75-aa region containing two predicted amphipathic alpha-helices. The KRAB domain is present in the amino-terminal regions of more than one-third of all Krüppel-class Cys2His2 zinc finger proteins and is conserved from yeast to man; however, its function is unknown. Here it is shown that the KRAB domain functions as a DNA binding-dependent transcriptional repressor when fused to a heterologous DNA-binding domain from the yeast GAL4 protein. A 45-aa segment containing one of the predicted KRAB amphipathic helices was necessary and sufficient for repression. Amino acid substitutions in the predicted helix abolished the repression function. These results assign a function, transcriptional repression, to the highly conserved KRAB box and define a minimal repression domain which may aid in identifying mechanisms of repression. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-05-10
