Table 1. Electrophoretic conditions for 10 enzymatic systems assayed for Gymnotus inaequilabiatus, G. sylvius and G in Genetic variability in three species of Gymnotus Linnaeus, 1758 (Gymnotiformes: Gymnotidae) from Caracu stream of the upper Paraná River basin, Brazil
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Table 1. Electrophoretic conditions for 10 enzymatic systems assayed for Gymnotus inaequilabiatus, G. sylvius and G. pantanal from Caracu stream, Paraná State, Brazil. EnzymeTissueBufferTimeReferencesAAT, EST, ADH, GDH, SODLiver, KidneyTris/borate/EDTA 0.045/0.025/0.001M, pH 8.67 hoursBoyer et al. (1963)G3PDH, LDH, SORBLiver, KidneyTris/citrate 0.009/0.003M, pH 7.014 hoursShaw & Prasad (1970)IDH, MDHLiver, Muscle, GillTris/citrate 0.009/0.003M, pH 7.014 hoursShaw & Prasad (1970)
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2009-12-18



