Effect of hypoxic stress on Estrogen receptor alpha binding to chromatin

This project aimed at assessing the influence of hypoxic stress on Estrogen receptor alpha genome wide binding to chromatin, in presence or absence of E2 ligand. I also aimed at assessing if a 1-month hypoxic stress followed by 1 month of normoxia imprints an epigenetic memory, therefore altering genome wide ER binding (rescue condition). 6 experimental conditions, with 1 technical replicate each 1) Normoxia : EtOH vs E2 (50 min); 2)Hypoxia: EtOH vs E2 (50 min); Rescue: EtOH vs E2 (50 min).