Strategy over 3 studies to obtain the strongest quality related genes on stored cumulus cells from ICSI patients.

The gene expression of cumulus cells (CC) related to different embryo morphology or pregnancy outcome of the corresponding oocytes in ICSI was assessed for three gene panels on three different sample sets. The genes found most predictive in each sample set were tested in the subsequent independent patient samples set. Genes marked in bold were retained as best predictive genes from the previous study. MII: metaphase II oocytes; COC: cumulus oophorus complex; rFSH: recombinant Follicle Stimulating Hormone; HP-hMG: Highly Purified human Menopausal Gonadotropin.