Effects of O-GlcNAcylation on VDJ recombination

We aimed to verify the effects of O-GlcNAcylation in VDJ recombination during early B cell development. We utilized a mouse model in which O-GlcNAc levels were downregulated by intraperitoneal administration of an O-GlcNAc Transferase (OGT) inhibitor (OSMI-1). To globally evaluate the sequence information utilized for rearrangement throughout the IgH gene locus, we conducted high-throughput genome-wide translocation sequencing-adapted repertoire sequencing (HTGTS-Rep-seq) using the genomic DNA from pro-B (B220+IgM-CD43) cells extracted from mouse bone marrow in the presence or absence of OSMI-1. The disruption of locus contraction and long-range interactions throughout the IgH locus depending on cellular O-GlcNAc levels were investigated by circular chromosome conformation capture and sequencing (4C-seq).