Ammonium excretion and respiration rate of tropical copepods and euphausiids measured during METEOR cruise M93, M97 and Maria S. Merian cruise MSM22@en

Respiration and ammonium excretion rates at different oxygen partial pressure were measured for calanoid copepods and euphausiids from the Eastern Tropical South Pacific and the Eastern Tropical North Atlantic. All specimens used for experiments were caught in the upper 400 m of the water column and only animals appearing unharmed and fit were used for experiments. Specimens were sorted, identified and transferred into aquaria with filtered, well-oxygenated seawater immediately after the catch and maintained for 1 to 13 hours prior to physiological experiments at the respective experimental temperature. Maintenance and physiological experiments were conducted in darkness in temperature-controlled incubators at 11, 13 or 23 degree C (±1). Before and during experiments, animals were not fed. Respiration and ammonium excretion rate measurements (both in µmol h-1 gDW-1) at varying oxygen concentrations were conducted in 12 to 60 mL gas-tight glass bottles. These were equipped with oxygen microsensors (ø 3 mm, PreSens Precision Sensing GmbH, Regensburg, Germany) attached to the inner wall of the bottles to monitor oxygen concentrations non-invasively. Read-out of oxygen concentrations was conducted using multi-channel fiber optic oxygen transmitters (Oxy-4 and Oxy-10 mini, PreSens Precision Sensing GmbH, Regensburg, Germany) that were connected via optical fibers to the outside of the bottles directly above the oxygen microsensor spots. Measurements were started at pre-adjusted oxygen and carbon dioxide levels. For this, seawater stocks with adjusted pO2 and pCO2 were prepared by equilibrating 3 to 4 L of filtered (0.2 µm filter Whatman GFF filter) and UV - sterilized (Aqua Cristal UV C 5 Watt, JBL GmbH & Co. KG, Neuhofen, Germany) water with premixed gases (certified gas mixtures from Air Liquide) for 4 hours at the respective experimental temperature. pCO2 levels were chosen to mimic the environmental pCO2 in the ETSP OMZ or the ETNA OMZ. […]本数据集针对采自东热带南太平洋（Eastern Tropical South Pacific, ETSP）与东热带北大西洋（Eastern Tropical North Atlantic, ETNA）的哲水蚤类桡足动物（calanoid copepods）和磷虾类（euphausiids），测定了不同氧分压下的呼吸速率与铵盐排泄速率。所有用于实验的标本均采自水柱上层400米水层，仅选取外观完好、状态健康的个体开展实验。标本捕获后立即进行分拣、鉴定，并转移至装有过滤且充分充氧海水的水族箱中，在对应实验温度下预培养1至13小时后方可开展生理实验。培养与生理实验均在黑暗环境下的控温培养箱中进行，设置温度为11、13或23℃（±1℃）。实验前后及实验全程，所有实验动物均不喂食。以微摩尔每小时每克干重（µmol h⁻¹ gDW⁻¹）为统一单位，在不同氧浓度条件下开展呼吸速率与铵盐排泄速率测定，实验容器为12至60毫升的气密玻璃瓶。此类玻璃瓶内壁安装有直径3毫米的氧气微传感器（ø 3 mm, PreSens Precision Sensing GmbH, 雷根斯堡, 德国），可无创监测瓶内氧浓度。氧浓度数据读取采用多通道光纤氧气变送器（Oxy-4与Oxy-10 mini, PreSens Precision Sensing GmbH, 雷根斯堡, 德国），通过光纤连接至氧气微传感器测点正上方的瓶外壁。实验在预先调整好的氧分压（pO₂）与二氧化碳分压（pCO₂）条件下启动。具体制备方法为：取3至4升经0.2微米Whatman GFF过滤器过滤，并经Aqua Cristal UV C 5 Watt（JBL GmbH & Co. KG, 诺伊霍芬, 德国）紫外灭菌的海水，在对应实验温度下与预混气体（液化空气Air Liquide提供的认证混合气）平衡4小时，以此制备调整好pO₂与pCO₂的海水储备液。实验所用pCO₂水平需模拟东热带南太平洋缺氧区（Oxygen Minimum Zone, OMZ）或东热带北大西洋缺氧区的环境pCO₂水平。[…]