five

Tn-Seq Survival Profiling

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE147760
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资源简介:
A Tn5 transposon insertion library generated in a thyA- strain was selected in thymidine-free media and insertions were mapped and sequenced at different time points to look for changes in survival caused by gene disruptions The library was thawed and pre-grown for 2h in high thymidine, washed twice in 1x phosphate buffered saline (PBS), and placed in thymidine-free media. Samples were collected at three time points during the selection: “Start”, “0h”, and “3h”.
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2023-01-10
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