Transcriptional response to latrunculin B treatment in WT and sid2 mutants

To investigate the role of salicylic acid biosynthesis in transcriptional response to cytoskeleton disruption, we treated WT and sid2 plants (mutant deficient in ICS1) by latrunculin B for 24 h. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 biological replicates. Fully expanded mature rosette leaves (three per plant) of Col-0 or sid2, were treated by infiltration of 1 µM of latrunculin B (Sigma) or 0.01% DMSO (Sigma) by needleless syringe. After 24h, leaves were detached and were fixed in liquid nitrogen and total RNA was extracted using RNAeasy Mini kit (Qiagen).