Aging induces aberrant state transition kinetics in murine muscle stem cells

Label retaining and non-retaining muscle stem cells from young and aged H2B-GFP+/-;rtTA+/- were profiled by single cell RNA-seq at two timepoints 2 young (3 mo) and 2 aged (20 mo) H2B-GFP+/-;rtTA were labeled to track proliferative history by pulsing DOX in development (E10.5-E16.5). Label retaining and non-retaining cells were isolated from these animals by FACS. A set of cells from each animal was profiled immediately, while another set of cells was activated for 18 hours in vitro (10% HS, DMEM, culture plastic + sarcoma derived ECM) prior to profiling. Single cell RNA-seq was performed following the 10X Chromium Single Cell v2 kit.