Integration of transcriptome, small RNAs and degradome sequencing uncovers a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall. [miRNA-seq]

The study of paeoniaflorin biosynthesis in Paeonia lactiflora Pall.at a molecular level is scarce, and the regulatory network between small RNAs and mRNAs in paeoniaflorin biosynthesis has not been assessed. Here, we attempt to combine mRNA and miRNA expression data with degradome analysis to reveal the tissue-specific regulatory network of miRNAs and their targets in Paeonia, especially the complex network of paeoniaflorin biosynthesis.The results will advance our understanding of the miRNA-mediated molecular mechanisms of paeoniaflorin and monoterpenoids biosynthesis, and provide a valuable resource for further study on Paeonia. Overall design: Four tissues of root, leaf, flower and fruit, which showed different paeoniflorin acculumation were employed. The root, leaf and flower were collected at the blossom period and the fruit was collected at young fruit period, immediately frozen in liquid nitrogen and stored at -80?. Each tissue has two biological replicates. we did a transcriptome-wide study on different tissues of Paeonia by combining RNA, sRNA and degradome sequencing to uncover a tissue-specific regulatory network of paeoniflorin biosynthesis in Paeonia Lactiflora Pall.