Genome-wide analysis of long noncoding RNA and mRNA expression profile in A549 CSCs and non-CSCs

Lung cancer is the malignant tumor with the highest incidence in the world. Cancer stem cells (CSCs) has the characteristics of immortal proliferation, self-renewal, differentiation of stem cells and high invasion and migration capabilities. In recent years, the function of non-coding RNA (ncRNA) that is regarded as transcription noise has been gradually discovered which  can participate in the occurrence and development of tumors by regulating the stemness of cancer stem cells. Herein, we detected the differential expressed lncRNAs in A549 NANOG+ (CSCs) and A549 NANOG- (non-CSCs) cells. Our finding suggest the profiles of lncRNAs changed in CSCs compared with non-CSCs and provide new insight into molecular mechanism that may account for the lung adenocarcinoma. Microarray expression profiling of lncRNAs and mRNAs were conducted using RNA extracted from three A549 Nanog+ (CSCs)samples and three A549 Nanog- (non-CSCs) samples