Single-cell transcriptomic profiling of non-hematopoietic circulating cells in mid-gestational embryos

Combined with FACS and single-cell RNA sequencing technology, this study systematically illuminated remarkable cell types and gene expression characteristics of non-hematopoietic cells in the circulation of mid-gestational embryos at the level of single-cell transcriptome for the first time, providing a reference for comprehensively analyzing the coordinated regulation of cells in different tissues and organs during embryonic development. Overall design: We performed Smart-Seq scRNA-seq of 300 cells to firstly construct a molecular landscape of non-hematopoietic circulating cells in mid-gestational mouse embryos