Bulk transcriptome analysis of conditional MYT1L-mutant ESC-derived human induced neurons following Wnt and/or Notch inhibitor treatment.

We examined the effect of Wnt and/or Notch inhibition in the context of heterozygous MYT1L deficiency in human induced neurons. Increased expression of genes with MYT1L binding motifs and activation of non-neuronal genes were observed in human neurons along with deregulation of autism-associated risk genes upon depletion of MYT1L. Bulk RNA sequencing of Wnt and/or Notch inhibitor-treated MYT1L (+/flox) ESC-derived human induced neurons following cre-mediated depletion (+/-) compared to delta-cre transduced isogenic controls, 7 days after NGN2-mediated induced neurogenesis.