Protocols for marker-free gene knock-out and knock-down in Kluyveromyces marxianus using CRISPR/Cas9

This file contains the CRISPR targets predicted for every gene in the NBRC1777 genome, you can use it to design primers to target the gene you are interested in. There is one folder per gene. The 'sgRNAcas9_report' file contains a list of all the predicted sites, their position in the gene, and other extra information (e.g. GC content, off targets, etc..). The 'TargetSeq.fa' file contains the sequence of the gene targeted. For more information see http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100448