Gene expression profiling of prostate infiltrating hematopoietic cells in TRAMP mice subjected to immunotherapy

Goal of the analysis was to identify the mechansisms accounting fo the synergy of T cells redirected to the tumor associated large T antigen and T cells redirected to the Uty minor histocompatibility antigen Gene expression profiling on CD45+ cells isolated from the prostate of mice treated with Allo/Y, Tumor or Tumor+Allo/Y ACT at one week after T cell transfer, the time of acute prostate infiltration. Samples recovered from mice treated with the combined Tumor+Allo/Y ACT and sacrificed after 28 days were also included in the experimental design to take into account changes occurring over time. Three mice per group were used.