DNA methylation analysis of malignant and normal uveal melanocytes reveals novel molecular features of uveal melanoma

In order to address epigenetic regulation, NM and UM models were analyzed through OxBS sequencing pipeline (Cambridge Epigenetics). Whole genome DNA methylation analysis was carried out with a Cambridge Epigenetics kit (TrueMethyl kit) that corresponds to an oxidative bisulfite reaction, to identify and analyze only 5-methylcytosine (5-mC). This modification of bisulfite sequencing was found to be more robust in our samples, particularly for the normal melanocytes, most probably due to the abundance of melanin Overall design: Normal choroidal melanocytes, fresh sorted UM cells from MP41 and MP46 models were analyzed in simplicate