Frequency of Sp1 consensus binding sites in the promoters of genes deregulated by Sp1.

Transcription factor binding sites were searched among the first 2000 nucleotides of the promoter region of differentially expressed genes and non-regulated genes using PATTERNn service. Sp1 binding motifs: GGGGCGGGG and GG[G/T]G[C/T]GGG. STAT6 binding motif: TTCN4GAA.(a)Control list of non-regulated genes generated randomly as described in the experimental procedures.(b)Up-regulated genes from differentially expressed gene list (>1.3 fold).(c)Down-regulated genes from differentially expressed gene list ((d)Statistical significance calculated as described in the experimental procedures. P-values are the comparison of up-regulated vs control and down-regulated vs control.