Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications

To explore genetic profiles in the cardiac tissues fabricated by heart extracellular matrix (HEM) hydrogel and dynamic flow in microfluidic chip (Chip flow), we analyzed and compared differences in mRNA expression levels of the each cardiac tissue (No HEM-Plate static, HEM-Plate static, No HEM-Chip flow, HEM-Chip flow). Examination of mRNA expression levels in 4 different groups 1) fabricated without HEM hydrogel and cultured in static well plate 2) fabricated with HEM hydrogel and cultured in static well plate 3) fabricated without HEM hydrogel and cultured in microfluidic chip with flow 4) fabricated with HEM hydrogel and cultured in microfluidic chip with flow