Morphological changes in the adult queen after ingestion of miR-184 in royal jelly by the larvae.

Note: The method for the feeding experiment is described in “Materials and methods”. The Control and miR-184 groups consisted of queen larvae reared with royal jelly in queen cups. These were fed either 5 ul DEPC-treated water (Control group) or 5 ul miR-184 (100 ng/µl) in DEPC-treated water (miR-184 group), respectively, when they were 2 days (26∼32 hrs after hatching) and 3 days (50∼56 hrs after hatching) old. The Normal worker group consisted of worker bees collected from the experimental colony during the same season.