Absolute Quantification of the Living Skin Microbiome Overcomes relic DNA Bias and Reveals Site-Specific Patterns Across Individuals

Using flow-cytometry and metagenomics, coupled with an upstream pre-processing step to remove relic-DNA (DNA left behind from dead or dying cells) to determine the live population of the skin microbiome. Repository contains important feature tables, counts tables, sequencing preprocessing scripts and feature table analysis scripts.本数据集结合流式细胞术（flow-cytometry）与宏基因组学（metagenomics）技术，并配合上游预处理步骤以去除残留DNA（relic-DNA，即死亡或濒死细胞遗留的DNA），从而解析皮肤微生物组（skin microbiome）的活性菌群构成。该数据集存储库包含关键特征表（feature tables）、计数表（counts tables）、测序预处理脚本（sequencing preprocessing scripts）以及特征表分析脚本（feature table analysis scripts）。