Integrative transcriptomic profiling of a mouse model of hypertension-accelerated diabetic kidney disease

Aim: To compare transcriptomic profiles of kidney cortex between healthy db/m mice, and mice with early stage diabetic kidney disease (uninephrectomized db/db injected with LacZAAV) and advanced stage diabetic kidney disease (uninephrectomized db/db mice injected with ReninAAV) Methods: Bulk RNA sequecing using the Illumina NextSeq 500 platform. Results: We identified 5,500 differentially expressed genes (DEGs) in db/db UNx LacZAVV mice compared to healthy controls, and 4,470 DEGs were identified in db/db UNx ReninAAV mice compared to healthy controls. Also, we showed in supplementery files that 3,039 DEGs were identified between db/db UNx LacZAAV mice and db/db UNx ReninAAV mice. Conclusion: We identified several gene expression changes in our two animal models of diabetic kidney disease. Overall design: Transcriptomic profiles of kidney cortex from healthy db/m mice, uninephrectomized db/db mice injected with a LacZ adeno-associated virus (AAV), and uninephrectomized db/db mice injected with AAV encoding renin to induce hypertension in the model.