Selected positions for the introduction of cysteines and calculated distances between the respective β-carbon atoms.

aPositions and residue indicated in parentheses for the 1st and 2nd amino acid (aa) corresponding to the five selected modifications (A to E). b Distances in Ångström (Å) between the two β-carbon atoms based on the crystal structures of full-length OspA from B. burgdorferi (serotype 1) bound to mAb 184.1 (N-terminal epitope), mAb LA-2 (C-terminal epitope) and a modified OspA [7], [34], [35].Selected positions for the introduction of cysteines and calculated distances between the respective β-carbon atoms.