Shh is a determinant of gammadelta T-cell development in the thymus

We compared gammadelta T-cell development and subsets in the thymus of mice in which Shh had been conditionally deleted from thymic epithelial cells using FoxN1-Cre to WT mice, and to transgenic mice in which a constitutively activator (Gli2DN2) or constitutuvely repressor (Gli2DC2) form of Gli2 were expressed under the control of the lck-promotor. We sorted CD27+CD3+TCR-gammadelta+ cells from 4 week old mice and comapred gene expression by RNAsequencing. All mice were on a C57BL/6 background. Wildtype, Gli2A, Gli2R and Shhfl/fl FoxN1 Cre+ (n=2) 4 week old thymi were sorted for CD3+CD27+gdTCR+ cells. RNA extracted from these cells was sequenced to help understand the transcriptional changes governing gd T cell development.