Data from: Quantitative proteomics reveals rapid divergence in the postmating response of female reproductive tracts among sibling species

Fertility depends, in part, on interactions between male and female
reproductive proteins inside the female reproductive tract (FRT) that
mediate postmating changes in female behavior, morphology, and physiology.
Coevolution between interacting proteins within species may drive
reproductive incompatibilities between species, yet the mechanisms
underlying postmating-prezygotic isolating barriers remain poorly
resolved. Here, we used quantitative proteomics in
sibling Drosophila species to investigate the
molecular composition of the FRT environment and its role in
mediating species-specific postmating responses. We found that
(1) FRT proteomes in D. simulans and D.
mauritiana virgin females express unique combinations of secreted
proteins and are enriched for distinct functional categories, (2) mating
induces substantial changes to the FRT proteome in D.
mauritiana but not in D. simulans, and (3)
the D. simulans FRT proteome exhibits limited postmating
changes irrespective of whether females mate with conspecific or
heterospecific males, suggesting an active female role in mediating
reproductive interactions. Comparisons with similar data in the closely
related outgroup species D. melanogaster suggest that
divergence is concentrated on the D. simulans lineage.
Our study suggests that divergence in the FRT extracellular environment
and postmating response contribute to previously described patterns
of postmating-prezygotic isolation and the maintenance
of species boundaries.