Transcriptional activity and epigenetic regulation of transposable elements in the symbiotic fungus Rhizophagus irregularis. Transcriptional activity and epigenetic regulation of transposable elements in the symbiotic fungus Rhizophagus irregularis

We report polyA+ mRNA profiles in a developmental assay where R. irregularis spores were exposed to either medium containing rice root-derived exudates or a mock nutrient medium. We report small RNA repertoires produced from R. irregularis spores using two different isolation techniques. We report the application of single-molecule-based DNA sequencing for profiling of CG methylation in untreated R. irregularis. spores. Overall design: Examination of mRNA profiles in mock and exudate-treated spores at three time points: a 0h untreated time point, 24h and 48h post treatment. RNAseq reads were generated by deep sequencing, in quadruplicate, using paired-end 150bp Illumina sequencing. Small RNA profiles were produced by oxidation (NaIO4 treatment) and Argonaute isolation (Lexogen TraPR column treatments), and compared to untreated controls.