An experimental test of the evolutionary consequences of sympatry in Drosophila subquinaria

Prezygotic isolation is often stronger between sympatric as opposed to allopatric taxa, but the underlying cause can be difficult to infer from comparative studies alone. Experimental evolution, where evolutionary responses to treatments manipulating the presence/absence of heterospecific individuals are tracked, can provide a powerful complementary approach. We used experimental evolution to investigate a naturally occurring pattern of reproductive character displacement in the mushroom-feeding fly, Drosophila subquinaria. In nature, female D. subquinaria from populations sympatric with the closely related D. recens discriminate more strongly against heterospecific males than do females from allopatric populations. Starting with 16 replicate allopatric populations of D. subquinaria, we manipulated the presence/absence of D. recens during mating (experimental sympatry vs. control) and, when present, we allowed hybrids to live or killed them each generation. Across 12 generations, hetero..., , , # An experimental test of the evolutionary consequences of sympatry in Drosophila subquinaria

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Uploaded data for a multi-generation evolution experiment testing the effect of artificial sympatry between two drosophila species. Data includes measures of mating rates and CHCs (cuticular hydrocarbons, a kind of contact pheromone) at each generation in each population.

## Description of the data and file structure

data_cons.csv <- contains conspecific mating rates from no-choice mating trials conducted in each population at each sampling generation (0,6, and 12). Columns are as follows: generation (sampling generation), block (experimental block where populations were placed in block 1 or block 2), blockXgen (factor for statistical methods concatonating block and generation), treatment (experimental treatment; Allo treatment = control, Str reinforcement = Hybrids killed, Wk reinforcement = Hybrids live), popID (population ID), male mated with (indicati...