Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with DADMe-ImmG and phosphate

Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with DADMe-ImmG and phosphate Descriptor: 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one, PHOSPHATE ION, Purine nucleoside phosphorylase Authors: Haapalainen, A.M, Ho, M.C, Suarez, J.J, Almo, S.C, Schramm, V.L. Deposit date: 2012-03-22 Release date: 2013-02-06 Last modified: 2024-10-30 Method: X-RAY DIFFRACTION (1.7 Å) Cite: Catalytic Site Conformations in Human PNP by (19)F-NMR and Crystallography. Chem.Biol., 20, 2013

人类来源的嘌呤核苷磷酸化酶（purine nucleoside phosphorylase）突变体（W16Y、W94Y、W178Y、H257W）与DADMe-ImmG及磷酸盐结合形成的复合物的晶体结构。配体描述：2-氨基-7-{[(3R,4R)-3-羟基-4-(羟甲基)吡咯烷-1-基]甲基}-3,5-二氢-4H-吡咯并[3,2-d]嘧啶-4-酮、磷酸根离子、嘌呤核苷磷酸化酶。作者：Haapalainen, A.M.、Ho, M.C.、Suarez, J.J.、Almo, S.C.、Schramm, V.L.。沉积日期：2012-03-22。发布日期：2013-02-06。最后修改日期：2024-10-30。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.7 Å。引用：《通过(19)F核磁共振波谱与晶体学解析人类嘌呤核苷磷酸化酶的催化位点构象》，《化学生物学》（Chem. Biol.），第20卷，2013年。