Time series single-nuclei RNA sequencing experiments of the early stages of Arabidopsis flower development

To understand the gene expression dynamics of flower development in the early stages at single cell level, we performed time series single-nuclei RNA sequencing (snRNA-seq) experiments using Arabidopsis thaliana flowers of developmental stages. Nuclei were isolated from pAP1:AP1-GR ap1-1 cal-1 Arabidopsis thaliana flower tissues after 0, 2, 4, 8 days of DEX induction. After DAPI staining, nuclei were collected by fluorescence-activated cell sorting (FACS) based on the DAPI peaks. SnRNA-seq library were then generated by SMARTer ICELL8 single-cell system