Transcriptional profiing of quiescent and lipopolysaccharide-stimulated HIVEP1 deficient pro-monocytic cells

In this study, we sought to broaden our understanding of HIVEP1 function in the cellular response to lipopolysaccharide (LPS). The study was done in the context of CRISPR-Cas9 knockdown of HIVEP1 in the pro-monocytic THP1-MD2-CD14 cell line. Overall design: Examination of RNA expression in THP1-MD2-CD14 cells deficient for HIVEP1 and controls with LPS (100 ng/ml) or medium for 2 or 8 hours. Sample legend: C2MED0h = control cells in RPMI medium (baseline); D2MED0h = HIVEP1 deficient cells in RPMI medium (baseline); C2MED2h = control cells in RPMI medium for 2hours; C2LPS2h = control cells in RPMI+LPS for 2 hours; C2MED8h = control cells in RPMI medium for 8hours; C2LPS8h = control cells in RPMI+LPS for 8 hours; D2MED2h = HIVEP1 deficient cells in RPMI medium for 2hours; D2LPS2h = HIVEP1 deficient cells in RPMI+LPS for 2 hours; D2MED8h = HIVEP1 deficient cells in RPMI medium for 8hours; D2LPS8h = HIVEP1 deficient cells in RPMI+LPS for 8 hours;