Transcriptome analysis of hypothalamuses from prepubertal and pubertal rats

We applied RNA sequencing analysis to hypothalamuses from prepubertal and pubertal rats. The significant differential expression transcripts were screened out. In order to validate the RNA-seq data, the expression patterns in the samples of lncRNAs and mRNA genes were confirmed by qRT-PCR. This research provides an expression profile of lncRNAs to further research on puberty. Overall design: LncRNA profiles of prepubertal and pubertal rats were generated by deep sequencing, three hypothalamuses for each group, using Illumina.