The Effect of 10% ointment concentration of ethyl acetate subfraction of Meniran (Phyllanthus niruri L.) leaves on excision wound healing in white male rats

<strong>Abstract</strong> <strong>Background: </strong>Wounds are skin problems that are often experienced by humans. Effective wound healing requires a complex arrangement of various healing processes that occur continuously. The plant that has been studied to have a role in the wound healing process is the herbal extract of meniran (<em>Phyllanthus niruri</em> L). This plant can be found in almost all parts of Indonesia. However, biomedical evidence related to the effect of giving meniran (<em>Phyllanthus niruri</em> L) leaves ethyl acetate subfraction ointment preparations is still not revealed yet. <strong>Methods: </strong>This study was carried out using experimental animals, a total of 27 rats were divided into 3 large groups, each group consisted of 9 rats, where group 1 was the control based ointment, group 2 was the comparison group (ointment T®) and group 3 was treated with 10% concentration of meniran leaf ethyl acetate subfraction ointment. Each group was observed and measured for three parameters, namely, percentage of wound healing, epithelialization time, and hydroxyproline levels. <strong>Results: </strong>From the results of data analysis using one-way (ANOVA) followed by the duncan test (SPSS 23.0) for epithelialization time and hydroxyproline levels, the results showed that 10% concentration of meniran leaf ethyl acetate subfraction ointment, the comparison group (T® ointment) and the control based group with the treatment group on the parameters of epithelialization time and hydroxyproline have levels significant of (p &lt;0.05) <strong>Conclusions: </strong>It can be concluded that the ethyl acetate subfraction ointment of meniran leaves with a concentration of 10% is effective in the healing process of the excision wound. <strong>Keywords</strong> <em>Phyllanthus niruri </em>L, wound healing , epithelialization, hidroksiprolin