Additional file 2 of Candidate Phyla Radiation (CPR) bacteria from hyperalkaline ecosystems provide novel insight into their symbiotic lifestyle and ecological implications

Additional file 2 : Table S1. Sampling and sequencing information for metagenomic data used in this study, including geographic locations, sequencing platform, data size, NCBI accession numbers, and source references. Table S2. List of bacterial strains and plasmids used in this study. Table S3. Characteristics for 38 MAGs, including quality, taxonomic assignment based on GTDB taxonomy. Table S4. Average amino acid identity (AAI) between our reconstructed CPR MAGs and reference CPR genomes from GTDB. Table S5. List of genes assigned to metabolic features of the 12 CPR MAGs. Table S6. Co-occurrence network showed that positive connections between CPR and non-CPR MAGs detected in this study. Table S7. Relative abundance (expressed as TPM values) and genome coverage for the hyperalkaline MAGs in each metagenome. Table S8. Presence and absence of the genes associated with element cycling (e.g., C, N, S, and O). Table S9. The index of replication (iRep) values for the MAGs reconstructed in the metagenomes. Table S10. Immediate genomic context for the scaffold harboring folA or thyA of the CPR bacteria and their potential THF-auxotrophic hosts. Table S11. The distribution of the folA and thyA genes in all Thermodesulfovibrionales (n = 226) and CSP1-3 (n = 44) genomes from GTDB (Release 220). Table S12. Potential transporters of folate molecules in the genomes of CPR and their auxotrophic hosts. Table S13. Classification and metabolic potential of folate cofactors of CPR genomes from this study and GTDB database.