Co-occurrence of mcr-1 and mcr-3 in E.coli isolated from healthy humans and pigs in Thailand

Rectal swabs were collected from 839 pigs (164-pooled samples) from 164 pig farms. Fecal samples were provided by from 159 farmers and 114 humans in proximity to farms but without direct contact with pigs. Isolation of multi-drug resistant (MDR) E. coli was done by use of selective methods. Minimum inhibitory concentrations and whole-genome sequencing (WGS) were performed for all isolates. High prevalence of mcr-positive E. coli including the evidence of coexistence with blaCTX-M gene indicated high MDR bacteria in pig and human origins in Thailand.