PBL Barley and AtPBS1 Alignment

Alignment of PBS1 and PBS1-like protein kinase amino acid sequences from barley and Arabidopsis, aligned by Clustal Omega. Barley sequences were obtained by a BLAST search using Arabidopsis PBLs as queries. This alignment was used to generate the phylogenetic tree in Figure 2A and S1 in Carter and Helm, et al 2018 (in submission).From methods section:Sequence similarity searches were performed using BLASTp to gather barley amino acid sequences homologous to Arabidopsis PBS1 and PBS1-like proteins. First, AtPBL (1 to 27), BIK1, and other PBS1-homologous sequences were gathered by searching the Arabidopsis genome (TAIR10, GCA_000001735.1) with the AtPBS1 (OAO91748.1) amino acid sequence and by name search. Potential barley PBLs were collected by searching the barley protein database (assembly Hv_IBSC_PGSB_v2) with each Arabidopsis homologue and taking the top five hits derived from distinct genes. Amino acid sequences were aligned with Clustal Omega (Sievers et al., 2011).