Bach2 negatively regulates natural killer cell maturation and effector program

Bach2 has been reported to regulate multiple immune cell development and function. Here we report that Bach2 is constitutively expressed in relatively immature NK subsets. Bach2 negatively regulates NK cell development and Bach2 deficiency results in NK cell with mature effector phenotype. Thus, Bach2 functions as a negative regulator of NK cell maturation and function. Overall design: Fresh NK cells from spleen of Bach2-conditional knockout mice or wild-type mice were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies). CD3-CD19-NK1.1+NKp46+ NK cells were then sorted from enriched cells. Total RNA were isolated by PureLink RNA Mini Kit (Ambion) and subjected to RNA-sequencing. The library was generated using low input Clontech method and NovaSeq 6000 was used for sequencing.