Single-cell RNA sequencing (scRNA-seq) of all IL-23R+ cell types in the small intestine of healthy mice

Single cell RNA-sequencing on all IL-23 receptor expressing cells in the intestine, revealing a dominance of lymphocytes including T cells and group 3 innate lymphoid cells (ILC3s). For scRNA-seq, IL-23R+ immune cells were sorted from small intestine of IL-23R-eGFP KI (JAX 035863) mice and stimulated with recombinant mouse IL-23 for 2 hours.