Gene Expression Analysis From Rat Models of Premature Ovarian Insufficiency Treated with Intraovarian Injection of Platelet Rich Plasma

Premature ovarian insufficiency (POI) has become a focus of many reproductive studies recently due to its long-life menopause-like symptoms in young women. It may affect up to three of a hundred women under 40 years. The possible causes include genetic diseases, infections, autoimmune disorders and iatrogenic events such as radiotherapy and chemotherapy. Recent studies investigating the regenerative dynamic potential of the ovaries demonstrate that follicular growth could be stimulated when an adequate ovarian environment is restored. This restoration can be achieved employing factors of known regenerative potential such as stem cells, or growth factors of platelet rich plasma (PRP), artificial ovary, ovarian transplantation, and mitochondrial replacement therapy. The emergence of autologous platelet-rich plasma (PRP) therapy reflects a break-through for POI patients, since the approach is the most ethical-friendly, the cheapest, the least invasive and limited adverse effects. Therefore, it has the highest potential to be applied in clinical routine practice. Nevertheless, the complex mechanism of PRP components' on the ovary, especially in term of folliculogenesis, remains to be deciphered. Furthermore, it is necessary to clarify to what extent they impact the gene or protein targets that play important role in ovarian rejuvenation after POI. We report raw fastq data of Sprague Dawley rat models of POI in four treatment groups and one control to evaluate the effect of intraovarian injection of PRP on POI rat models. This report is a part of animal study regarding the effect of intraovarian injection of PRP on ovarian function recovery in POI rat models (unpublished study). The data collected from this study will be used to investigate how PRP induce the ovarian rejuvenation and to analyse transcriptomic changes after PRP treatment on chemotherapy-induced POI animal models. Overall design: Young adult of female Sprague Dawley rats (10-12 weeks) were used as animal models. Each group consisted of 6 rats. Serial injections of Cisplatin (3 mg/kg body weight, intraperitoneal, once a week) were performed either three- or five-time to induce mild- or severe-POI rat models, while control group was injected with NaCl 0.9%. Intraovarian injection of PRP (processed from the donor rats' blood) was conducted on one mild- and severe-POI group a week after last injection of cisplatin, through laparotomic procedure. Post-operative infection was controlled with antibiotics (for the first three days). All samples for the study were collected two weeks after PRP treatment, and two weeks after last injection for control group. From each group, one ovarian tissue was randomly selected to involve in this transcriptomic analysis. The samples were stored in RNA Later solution (-80oC) before study procedure. Detailed research procedures were in accordance with the main study regarding the effect of intraovarian injection of PRP on ovarian function recovery in POI rat models (Roza et al, unpublished study).