The effect of BzpN on spore viability.

107 cells were allowed to develop on filter pads and spores were collected in buffer by repeated washing of the filter pad using a pipette. The density of visible spores in buffer was determined by hemocytometer and the total number of collected spores was calculated. Spores were then treated with detergent, and serial dilutions of detergent-treated spores were plated on SM/5 plates in association with bacteria. The number of resultant plaques in the bacterial lawn was used to calculate the total number of detergent resistant spores as a percent of input cell number. Values are the mean ± SEM from three independent experiments.